Improved Structure and Development of Bone using Biofield Energy Enriched Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63)
The present study aimed to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters in MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. Both the samples received Consciousness Energy Healing Treatment by Maria Isabel Aguilar Tiraboschi and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 191.7% (0.1µg/mL), 127.8% (at 1µg/mL), and 136.1% (at 10µg/mL) in BT-DMEM+UT-TI, BT-DMEM+BT-TI, and UT-DMEM+BT-TI groups respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 267.9%, 128.9%, and 91.8% at 1, 10, and 50µg/mL, respectively in UT-DMEM+BT-TI group, while 169.6% at 1µg/mL in BT-DMEM+UT-TI group respectively as compared with the untreated group. Besides, BT-DMEM+BT-TI group showed an increased collagen value by 155.4% and 100% at 1 and 10 µg/mL respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 139.8%, 91%, and 44.7% at 0.1, 10, and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 126.7%, 79.3%, and 67.5% at 0.1, 10, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 31.3%, 33.2%, and 152.5% at 0.1, 10, and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.